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. 2014 Sep 11;155(12):5024–5032. doi: 10.1210/en.2014-1316

Figure 5.

Figure 5.

Sox3 is expressed in the proliferating adult epithelial cells at the climax of intestinal remodeling. A, Intestinal sections of X. laevis tadpoles at the premetamorphic stage (stage 54), the climax (stage 61), or the end (stage 66) of metamorphosis were analyzed by in situ hybridization with digoxigenin-labeled Sox3 RNA probes and visualized with AP-linked antidigoxigenin antibody and BCIP/NBT staining. B, Serial sections of the intestine of X. laevis tadpoles at the climax (stage 62) were analyzed by in situ hybridization with digoxigenin-labeled Sox3 RNA or LGR5 probes, respectively, and visualized with AP-linked anti-digoxigenin antibody and BCIP/NBT staining. Note that Sox3 expressing cells largely overlapped with those ones expressing LGR5. Arrows indicate cell clusters expressing both Sox3 and LGR5. Abbreviations: Ct, connective tissue; Lu, lumen; M, muscle; Ty, typhlosole. Scale bar, 100 μm.