TABLE 1.

Protein-DNA affinities and thermal stability

FRET-based estimates of dissociation constant (K_d).

Variant	Kd (nm) 15 °C	Kd (nm) 37 °C	Tm (complex) (°C)a
WT	14 ± 3	14 ± 2	62
W70A	53 ± 6	259 ± 37	44
W70L	330 ± 30	466 ± 46	42
W70F	49 ± 5	24 ± 2	59
W70Y	32 ± 3	26 ± 4	59

^a T_m is given at a protein-DNA concentration of 25 μm.