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Comparison of the kinetic constants of wild type P450revI and several site-specific mutants for the substrate RM-T
The wild type kcat/Km value of 372 min−1 μm−1 was considered 100%.
| Enzyme | Km | kcat | kcat/Km | Relative activity |
|---|---|---|---|---|
| μm | min−1 | min−1 μm−1 | % | |
| Wild typea | 0.89 ± 0.05 | 331 ± 5 | 372 | 100 |
| L287F | 5.58 ± 0.82 | 89.5 ± 3.5 | 16.0 | 4.3 |
| F91G | 12.5 ± 2.0 | 197 ± 11 | 15.8 | 4.2 |
| A292V | 13.1 ± 1.1 | 143 ± 3 | 10.9 | 2.9 |
| R81A | 75.2 ± 9.4 | 84.4 ± 5.2 | 1.12 | 0.3 |
| R190A | NAb | NA | NA |
a Data are from Table 2.
b NA, no activity.
