FIGURE 8.

Effect of α-TOS on peroxidase activity of rat liver mitochondria. Mitochondria (0.9 mg of protein/ml) were incubated with liposomes containing DOPE/sphingomyelin/α-TOS (9:2:1) in 100 μl of MIB buffer without EGTA for 45 min at 37 °C. Peroxidase activity was determined in the presence of Amplex Red (50 μm) and tBOOH (2 mm), by measuring the fluorescence of resorufin, an oxidation product of Amplex Red. The final concentration of P2 fraction was 0.25 mg of protein/ml. Data are mean ± S.D., n = 3; *, p < 0.05 versus control.