FIGURE 2.

Enrichment of glycoproteins identified by mass spectrometry in EMV. Confirmation of the enrichment of select proteins in Sk-Mel-5 EMVs. Equal amounts of TCM and isolated EMVs (5 μg of protein each) were loaded onto SDS-PAGE gels and analyzed by Western blot analysis with the appropriate antibodies. Equal loading of protein was confirmed by Ponceau staining (data not shown). Samples were analyzed for EMV markers CD81 and CD63 as a control. Western blots are representative of three biological replicates. A, α-EWI-2. B, α-G3BP; C, α-ADAM10. D, α-CD81. E, α-CD63.