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. 2014 Sep 26;289(47):32526–32537. doi: 10.1074/jbc.M114.606269

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Glycosylation alters galectin-3 and G3BP recruitment to EMV. Sk-Mel-5 cells were treated with DMJ (1 mm) for 48 h before collection of TCM or EMV (+ DMJ). Untreated Sk-Mel-5 cells were used as a control (− DMJ). A, TCM and EMV samples (+/− DMJ) were probed for galectin-3. Equal amounts of TCM and EMV were loaded in each lane (5 μg of protein) and analyzed by SDS-PAGE and Western blot analysis. B, TCM and EMV samples (+/− DMJ) were probed for G3BP. Equal amounts of TCM samples (25 μg) and of EMV (10 μg) were compared by SDS-PAGE and Western blot analysis. The position of glycosylated G3BP is indicated by arrows. Western blots shown are representative of two biological replicates.