TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli | ||
| Keio collection (parent strain, BW25113) | F−, DE(araD-araB)567 lacZ4787(del)::rrnB-3 LAM− rph-1 DE(rhaD-rhaB)568hsdR514 | (21) |
| YS Collection 7a | ΔemrB ΔacrB::KmR | This work |
| YS Collection 7b | ΔemrB ΔacrB ΔacrE::KmR | This work |
| YS Collection 7c | ΔemrB ΔacrB ΔemrY::KmR | This work |
| YS Collection 7d | ΔemrB ΔacrB ΔmacB::KmR | This work |
| YS14530 | ΔmarRA::CmR | This work |
| YS13007 | ΔtatC | (4) |
| YS15910 | ΔtolC ΔtatC | This work |
| YS15924 | ΔdegP ΔdsbA | This work |
| YS15925 | ΔtolC::KmR ΔdegP ΔdsbA | This work |
| YS15926 | ΔtatC::KmR ΔdegP ΔdsbA::KmR | This work |
| YS15537 | tolC-lac::KmR | This work |
| YS15538 | tolC-lac::KmR cpxR | This work |
| PND2000 | MC4100, kRS88[degP-lacZ] | (42) |
| SP1 | MC4100 ara +λplacMu53[cpxP-lacZ] | (43) |
| MG1301 | MC4100mgrB::λplacMu55 | (44) |
| DH5α | F− supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | (New England Biolabs) |
| BL21-Gold (DE3) | E. coli B F− ompT hsdSB (rB− mB−) dcm+ Tetr gal λ(DE3) endA Hte | (Agilent Technologies) |
| Plasmids | ||
| pKD4 | repR6K γApR FRT KmR FRT | (22) |
| pKD46 | reppSC101ts ApR ParaBAD γ β exo | (22) |
| pCP20 | reppSC101ts ApR CmR cI857 λPR | (22) |
| pKG136 | repR6Kγ KmR FRT lacZY this | (23) |
| pACYC184 | repp15A CmR TcR | (New England Biolabs) |
| pUHE21–2lacq | reppMB1 ApR lacIq | (24) |
| pYS2132 | reppMB1 ApR lacIq nlpE | (4) |
| pYS1755 | reppMB1 ApR lacIq tolC | This work |
| pYS1000 | repp15A CmR Plac1–6 lacZ this | (25) |
| pYS1736 | repp15A CmR Plac1–6 Pmar | This work |
| pET28a | repColE1 KmR lacI PT7 | EMD Biosciences |
| pYS2211 | repColE1 KmR lacI PT7 (Eco) cpxR-His6 | This work |