qRT-PCR |
MAT1A-F |
AGAGTGCTTGTCCAGGTT |
97 |
MAT1A-R |
GCTCTCGCTCTGTCTTCT |
GRE1-F |
TCAGAATACAGGTGCGTGCT |
74 |
GRE1-R |
CTGCGTCTCATCTGGATTGGT |
GRE2-F |
ATTCCCCATTGTTCCTTGGGT |
70 |
GRE2-R |
TGTACTAAATGACAGCGTCTCACA |
HBV-GRE-F |
CTGGCCAAAATTCGCAGTCC |
84 |
HBV-GRE-R |
GACACATCCAGCGATAGCCA |
GAPDH-F |
CAAGTTCAACGGCACAGTCA |
100 |
GAPDH-R |
CCATTTGATGTTAGCGGGAT |
|
|
Truncation |
pMAT1A1.4Luc-F |
CGCACGCGTTTCCAGAAGAGGTCACCTTAATACT |
1474 |
pMAT1A1.2Luc-F1 |
CGCACGCGTAGTCCAAGCTTTGATGCACAAGGTT |
1274 |
pMAT1A0.9Luc-F2 |
CGCACGCGTAAACTGGACTTTGATAATTTCCCTG |
974 |
pMAT1A0.8Luc-F3 |
CTCACGCGTACCTCCCCAGATAGATACTTTTTAA |
874 |
pMAT1ALuc-R |
ATACTCGAGCTTCTCACACTTCTCCACTCACGCT |
|
|
Mutation |
del−876 to −862-F4 |
TAAAAAAAAAACACACACACACACTGTAACCTCCCCAGATAGATAC |
|
del−876 to −862-R2 |
GTATCTATCTGGGGAGGTTACAGTGTGTGTGTGTGTTTTTTTTTTA |
|
del−1022 to −1008-F5 |
TCAAAGCAAAAAATAATGCAAGAGTTATGTTACATGTATAGAACTGAATATGTAGATG |
|
del−1022 to −1008-R3 |
CATCTACATATTCAGTTCTATACATGTAACATAACTCTTGCATTATTTTTTGCTTTGA |
|
MUT CpG1-F |
CAGCTGGATCTGAGAGTGTGAGAAGCTGTCATTTAG |
|
MUT CpG1-R |
CTAAATGACAGCTTCTCACACTCTCAGATCCAGCTG |
MUT CpG2-F |
CAAAAAATAATGCAAGAGTTATGTGAACAAGATGTTTATTACATGTATAGAACTGAATA |
|
MUT CpG2-R |
TATTCAGTTCTATACATGTAATAAACATCTTGTTCACATAACTCTTGCATTATTTTTTG |
MUT CpG3-F |
GTATAGAACTGAATATGTAGATGTTTATAATACGGAAGCTTTGGGAAA |
|
MUT CpG3-R |
TTTCCCAAAGCTTCCGTATTATAAACATCTACATATTCAGTTCTATAC |
MUT CpG4-F |
GTTTCTGTTAGCAGAAACAAGTGGACTCAAAGCTTTTCC |
|
MUT CpG4-R |
GGAAAAGCTTTGAGTCCACTTGTTTCTGCTAACAGAAAC |
|
|
ChIP |
chip-GRE1F |
CACATTGTTCTCTGTAACCTCCCCA |
254 |
chip-GRE1R |
TCCCCTGCGTCTCATCTGGATTGGT |
chip-GRE2F |
ATTCCCCATTGTTCCTTG |
154 |
chip-GRE2R |
AACATCGTGTTCACATAACT |
chip-HBV-GRE-F |
CTCGTGTTACAGGCGGGGTTTTTCT |
206 |
chip-HBV-GRE-R |
ATAAAACGCCGCAGACACATCCAGC |
|
|
MSP |
MAT1A-F |
AGGAAGAGAGTTGTATTTTGAGTTAGTTGTGATGTT |
|
MAT1A-R |
CAGTAATACGACTCACTATAGGGAGAAGGCTCAATTCCCCATTATTCCTTAAATCT |
|