TABLE 1.
DNA sequences of primers used in the study
qRT-PCR is quantitative RT-PCR; F is forward; R is reverse.
| Primer name | Sequence (5′ to 3′) | bp |
|---|---|---|
| qRT-PCR | ||
| MAT1A-F | AGAGTGCTTGTCCAGGTT | 97 |
| MAT1A-R | GCTCTCGCTCTGTCTTCT | |
| GRE1-F | TCAGAATACAGGTGCGTGCT | 74 |
| GRE1-R | CTGCGTCTCATCTGGATTGGT | |
| GRE2-F | ATTCCCCATTGTTCCTTGGGT | 70 |
| GRE2-R | TGTACTAAATGACAGCGTCTCACA | |
| HBV-GRE-F | CTGGCCAAAATTCGCAGTCC | 84 |
| HBV-GRE-R | GACACATCCAGCGATAGCCA | |
| GAPDH-F | CAAGTTCAACGGCACAGTCA | 100 |
| GAPDH-R | CCATTTGATGTTAGCGGGAT | |
| Truncation | ||
| pMAT1A1.4Luc-F | CGCACGCGTTTCCAGAAGAGGTCACCTTAATACT | 1474 |
| pMAT1A1.2Luc-F1 | CGCACGCGTAGTCCAAGCTTTGATGCACAAGGTT | 1274 |
| pMAT1A0.9Luc-F2 | CGCACGCGTAAACTGGACTTTGATAATTTCCCTG | 974 |
| pMAT1A0.8Luc-F3 | CTCACGCGTACCTCCCCAGATAGATACTTTTTAA | 874 |
| pMAT1ALuc-R | ATACTCGAGCTTCTCACACTTCTCCACTCACGCT | |
| Mutation | ||
| del−876 to −862-F4 | TAAAAAAAAAACACACACACACACTGTAACCTCCCCAGATAGATAC | |
| del−876 to −862-R2 | GTATCTATCTGGGGAGGTTACAGTGTGTGTGTGTGTTTTTTTTTTA | |
| del−1022 to −1008-F5 | TCAAAGCAAAAAATAATGCAAGAGTTATGTTACATGTATAGAACTGAATATGTAGATG | |
| del−1022 to −1008-R3 | CATCTACATATTCAGTTCTATACATGTAACATAACTCTTGCATTATTTTTTGCTTTGA | |
| MUT CpG1-F | CAGCTGGATCTGAGAGTGTGAGAAGCTGTCATTTAG | |
| MUT CpG1-R | CTAAATGACAGCTTCTCACACTCTCAGATCCAGCTG | |
| MUT CpG2-F | CAAAAAATAATGCAAGAGTTATGTGAACAAGATGTTTATTACATGTATAGAACTGAATA | |
| MUT CpG2-R | TATTCAGTTCTATACATGTAATAAACATCTTGTTCACATAACTCTTGCATTATTTTTTG | |
| MUT CpG3-F | GTATAGAACTGAATATGTAGATGTTTATAATACGGAAGCTTTGGGAAA | |
| MUT CpG3-R | TTTCCCAAAGCTTCCGTATTATAAACATCTACATATTCAGTTCTATAC | |
| MUT CpG4-F | GTTTCTGTTAGCAGAAACAAGTGGACTCAAAGCTTTTCC | |
| MUT CpG4-R | GGAAAAGCTTTGAGTCCACTTGTTTCTGCTAACAGAAAC | |
| ChIP | ||
| chip-GRE1F | CACATTGTTCTCTGTAACCTCCCCA | 254 |
| chip-GRE1R | TCCCCTGCGTCTCATCTGGATTGGT | |
| chip-GRE2F | ATTCCCCATTGTTCCTTG | 154 |
| chip-GRE2R | AACATCGTGTTCACATAACT | |
| chip-HBV-GRE-F | CTCGTGTTACAGGCGGGGTTTTTCT | 206 |
| chip-HBV-GRE-R | ATAAAACGCCGCAGACACATCCAGC | |
| MSP | ||
| MAT1A-F | AGGAAGAGAGTTGTATTTTGAGTTAGTTGTGATGTT | |
| MAT1A-R | CAGTAATACGACTCACTATAGGGAGAAGGCTCAATTCCCCATTATTCCTTAAATCT | |