TABLE 1.
Plasmids used in this study
| Plasmids | Description | Reference/Source |
|---|---|---|
| pRSVCATam1.2.5 trnfM U35A36/G72G73/QRS | pRSVCATam1.2.5 trnfM U35A36/G72G73 with glutaminyl-tRNA synthetase (QRS) gene; ColE1 origin; ampr | Ref. 55 |
| pACD | E. coli vector containing p15A origin of replication, tetr | Ref. 55 |
| pACD MTFs | pACD containing gene for E. coli MTF suppressor (MTFs) under lac promoter | Ref. 27 |
| pACD MTFs A89S | pACD MTFs with A89S mutation | This study |
| pACD MTFs A89L | pACD MTFs with A89L mutation | This study |
| pACD MTFs A172S | pACD MTFs with A172S mutation | This study |
| pACD MTFs A172L | pACD MTFs with A172L mutation | This study |
| pQE16 MTF | pQE16 expression vector (Qiagen) containing E. coli wild-type MTF gene; ampr | Ref. 27 |
| pQE16 MTF A89L | pQE16 MTF with A89L mutation | This study |
| pQE16 MTF A172L | pQE16 MTF with A172L mutation | This study |
| pET mt-MTF | pET19-based expression vector (Novagen) containing wild-type human mt-MTF; ampr | This study |
| pET mt-MTF S125L | pET mt-MTF with S125L mutation | This study |
| pET mt-MTF S209L | pET mt-MTF with S209L mutation | This study |