TABLE 2.

Steady state kinetic parameters of formylation of E. coli initiator tRNA₂^fMet using wild-type and mutant E. coli MTF enzymes

Kinetic parameters were measured using Lineweaver-Burk plots. The kinetic parameters listed are the average and S.D. of three independent measurements.

E. coli MTF	Km	Vmax	Vmax/Km	Approximate -fold decrease in Vmax/Kma
	μm	pmol·min−1·μg−1
Wild type	0.51 ± 0.08	4080.18 ± 635.91	8000.35	1
A89L	5.87 ± 2.06	326.78 ± 60.51	55.67	144
A172L	1.07 ± 0.39	1941.09 ± 180.17	1814.10	4

^a Approximate -fold decrease in V_max/K_m is the ratio of V_max/K_m of wild-type MTF to that of the mutant MTF, setting wild-type MTF to 1.