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. 2014 Oct 7;289(47):32757–32772. doi: 10.1074/jbc.M114.585901

FIGURE 1.

FIGURE 1.

Development of TLR-expressing derivatives of HEK293 cells (HEK293-TLR4/MD2 and HEK293-TLR2 cells) by stable transfection. A, LPS-dependent induction of luciferase activity. The HEK293-TLR4/MD2 cells were treated with each concentration of LPS (0–150 ng/ml) for 24 h. The results shown are means ± S.D. (error bars) of three independent experiments. B, effect of polymyxin B on LPS-induced luciferase activity. The HEK293-TLR4/MD2 cells were pretreated with each concentration of polymyxin B (0–1000 μg/ml) and then treated with 10 ng/ml LPS for 24 h. The results shown are means ± SD (error bars) of three independent experiments. C, Pam3CSK4-dependent induction of luciferase activity. The HEK293-TLR2 cells were treated with each concentration of Pam3CSK4 (0–100 ng/ml) for 24 h. The results shown are means ± S.D. (error bars) of three independent experiments. D, effect of anti-TLR2 neutralizing antibody on Pam3CSK4-induced luciferase activity. The HEK293-TLR2 cells were pretreated with each concentration of anti-TLR2 neutralizing antibody (0–20 μg/ml) and then treated with 2 ng/ml Pam3CSK4 for 24 h. The results shown are means ± S.D. (error bars) of three independent experiments.