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Replisome-mediated Pol IV-catalyzed TLS bypass requires interaction with β and TLS activity. Standard gyrase replication reactions containing either the CPD (A) or THF template (B), the indicated TLS polymerase at 100 nm, standard concentrations of dCTP and TTP, and 750 μm dATP and dGTP were incubated, processed, and analyzed as described under “Experimental Procedures.” ΔC5, Pol IV ΔC5; F13V, Pol IV F13V. Other abbreviations are as defined in the legend to Fig. 3.
