FIGURE 7.

Stalled nascent leading strand is chased into full-length product during Pol IV-catalyzed, replisome-mediated TLS bypass. A, scheme of the replication reaction. B and C, pulse-chase EcoRI replication reactions containing [α-³²P]dATP using either the THF (B) or CPD (C) template either in the presence or absence of Pol IV (100 nm) were incubated, processed, and analyzed as described under “Experimental Procedures.” Times are post chase. ERI, early replication intermediate; topo, topoisomerase. Other abbreviations are as defined in the legend to Fig. 3.