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. 2014 Oct 14;289(47):32871–32882. doi: 10.1074/jbc.M114.593897

TABLE 1.

Intra-molecular FRET efficiencies relative to Cys62 (α2)

E′ − preSTS E′ − postSTS
Glu17 − α1 (F′, 15 cells; FQ′, 15 cells) 0.31 ± 0.16a NFb
Arg78 − α3 (F′, 14 cells; FQ′, 14 cells) 0.43 ± 0.15 0.67 ± 0.07
Cys126 − α5c (F′, 16 cells; FQ′, 17 cells) 0.45 ± 0.14 0.11 ± 0.20
Asp154 − α7 (F′, 12 cells; FQ′, 13 cells) 0.49 ± 0.09 NF
Ile175 − α9 (F′, 15 cells; FQ′, 18 cells) NF NF

a Errors in FRET efficiency reported in this paper reflect the S.D. in the fluorescent intensity ratios.

b No FRET efficiency was detected between this position and Cys62 (α2).

c FRET efficiencies reported in Ref. 13.