TABLE 1.
E′ − preSTS | E′ − postSTS | |
---|---|---|
Glu17 − α1 (F′, 15 cells; FQ′, 15 cells) | 0.31 ± 0.16a | NFb |
Arg78 − α3 (F′, 14 cells; FQ′, 14 cells) | 0.43 ± 0.15 | 0.67 ± 0.07 |
Cys126 − α5c (F′, 16 cells; FQ′, 17 cells) | 0.45 ± 0.14 | 0.11 ± 0.20 |
Asp154 − α7 (F′, 12 cells; FQ′, 13 cells) | 0.49 ± 0.09 | NF |
Ile175 − α9 (F′, 15 cells; FQ′, 18 cells) | NF | NF |
a Errors in FRET efficiency reported in this paper reflect the S.D. in the fluorescent intensity ratios.
b No FRET efficiency was detected between this position and Cys62 (α2).
c FRET efficiencies reported in Ref. 13.