TABLE 1.

Nucleotide binding and oligomerization parameters of ClpB NBD1-M wild type, R322A, R323A, and R322A/R323A

^a The characterization of ClpB NBD1-M wild type has been published previously (25). The parameters are given here for comparison.

^b From stopped flow experiments.

^c From fluorescence equilibrium titrations.

^d Measurements were performed in the absence and presence of nucleotide. The molecular mass of monomeric NBD1-M is 45 kDa.

^e ATP-containing solutions were supplemented with phosphoenolpyruvate and pyruvate kinase as an ATP-regenerating system.