TABLE 2.

Nucleotide binding parameters of covalently linked ClpB NBD1-M dimer variants

See Fig. 3 for corresponding experimental data. Stopped flow experiments were performed at [NBD1-M] = 1 μm in the final mixture. Equilibrium titrations were performed at [NBD1-M] = 2 μm. n.d., not determined.

^a The terms “free” and “cross-linked” refer to the two different nucleotide binding sites, one being located in the free (outside) interface and the other one in the cross-linked (inside) interface.