TABLE 3.

ATPase activity parameters of covalently linked ClpB NBD1-M dimer variants

See Fig. 4, A, C, and E, for corresponding experimental data. The ATPase activity was calculated per monomer. The Hill equation was used for data fitting (Equation 1).

^a The characterization of ClpB NBD1-M wild-type has been published previously (25). The parameters are given here for comparison.

^b The data showed no sigmoidal shape and were therefore fitted using the Michaelis-Menten equation, which is represented by a simple hyperbolar function (Hill coefficient n = 1).