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. 2014 Oct 4;289(47):33044–33053. doi: 10.1074/jbc.M114.573857

FIGURE 5.

FIGURE 5.

Overexpression of CGI-58 promotes mitochondrial fission by up-regulating the expression of DRP-1. A, Western blot analysis of MFN2, OPA1, and DRP1 protein levels in C2C12 cells stably expressing CGI-58 or vector control. GAPDH was used as an internal control. B, confocal microscopic analysis of mitochondrial network in C2C12 cells stably expressing CGI-58 or vector control. To identify a role of CGI-58 in mitochondrial fission, C2C12 cells stably expressing CGI-58 were also treated for 4 h with 25 μm Mdivi-1, a mitochondrial division inhibitor, and analyzed for mitochondrial dynamics after staining with MitoTracker Red, a red fluorescent dye that stains mitochondria in live cells. Scale bar, 10 μm. C, analysis of the role for CGI-58 in mitochondrial fusion in C2C12 cells. C2C12 cells stably expressing CGI-58 or vector control were transiently transfected with expression plasmids for mito-EGFP or mito-DsRed, co-plated, and fused with PEG 1500, followed by confocal microscopic imaging analysis. Scale bar, 10 μm.