FIGURE 8.

CGI-58 overexpression and depletion conversely regulated AMPK and mTORC1 signaling in C2C12 cells. A, C2C12 cells stably expressing CGI-58 or vector control were stimulated with indicated doses of insulin for 15 min, followed by analysis of phosphorylation of Akt, AMPK, S6K1, and 4E-BP1 by Western blotting using antibodies for indicated proteins and their phosphorylated forms. GAPDH was used as an internal control. B, C2C12 cells stably expressing CGI-58 shRNA or vector control were stimulated with indicated doses of insulin for 15 min, followed by a similar analysis as indicated in A.