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. 2014 Oct 15;289(47):33054–33065. doi: 10.1074/jbc.M114.582304

FIGURE 1.

FIGURE 1.

Loss of KRIT1 and PDCD10, but not CCM2, stimulates nuclear β-catenin-dependent expression of Vegfa. A, knockdown efficiency of anti-KRIT1, CCM2, and PDCD10 siRNA on Krit1, CCM2, PDCD10, and Ctnnb1 (β-catenin) mRNA expression. B, β-catenin-dependent reporter activity in HPAEC transfected with NC, anti-KRIT1, -CCM2, and -PDCD10 siRNA. TOPFlash activity is shown relative to NC siRNA-transfected cells (±S.E.) and compared with cells overexpressing β-catenin [βcat]. n = 4, p = 0.0011 by ANOVA; *, p < 0.05 by post-hoc test versus NC-transfected cells. C, expression of Vegfa mRNA in siRNA-transfected HPAEC. Data shown are the average expression from three experiments, ± S.E., p = 0.0002 by ANOVA; *, p < 0.001 by post-hoc test versus NC-transfected cells. D, concentration of VEGF-A secreted from NC and KRIT1 siRNA-transfected cells. n = 9, p = 0.003 by ANOVA; *, p < 0.05 by post-hoc test versus NC-transfected cells; **, p < 0.01 versus KRIT1 siRNA-transfected cells. E, expression of Vegfa mRNA in siRNA-transfected cells co-expressing dn-TCF ±S.E; n = 3, p = 0.003 by ANOVA; *, p < 0.05 by post-hoc test versus NC-transfected cells; **, p < 0.01 versus KRIT1 siRNA-transfected cells.