Fig. 7.

Effects of extracellular LPC on fatty acid-stimulated oxidation in isolated hepatocytes. Primary mouse hepatocytes were plated at 5000 cells/well in a collagen-coated 96-well Seahorse plates and treated with 50 μM oleate with or without the indicated concentrations of LPC at 15 min (A). Oligomycin (10 μg/mL) was added at 30 min (B), 3 μM FCCP was added at 40 min (C), and 4 μM antimycin A/1 μM rotenone were added at 65 min (D). Panel A shows representative tracings at each LPC concentration. Panel B shows mean data ± standard deviations from 3-6 separate determinations. Panel C shows baseline oxygen consumption rate (OCR) prior to addition of oleate and LPC. Fatty acid induced rate was determined by subtracting basal OCR from the increasein OCR following addition of oleate or oleate/LPC. Maximal respiration was determined following addition of FCCP. Reserve capacity was determined by the remaining OCR following administration of oligomycin. Non-mitochondrial respiration was determined from the respiration following administration of antimycin A and rotenone. Data represent mean ± standard error. * denotes significant differences from samples without LPC addition at P < 0.05.