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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Neurobiol Learn Mem. 2013 Nov 27;107:93–100. doi: 10.1016/j.nlm.2013.11.008

Figure 2. Intra-LA infusion of an inhibitor of DNMT activity impairs fear memory reconsolidation and retention of memory-associated neural plasticity in the LA.

Figure 2

(a) Rats were habituated and given two days of baseline AEFP recording sessions, followed by fear conditioning with 3 tone pip series-(CS) shock (US) pairings. Twenty four hrs following training rats were given a memory reactivation session consisting of a single tone pip series CS presentation followed 1 hr later by intra-LA infusion of vehicle (n = 6), RG108 (500 ng/side; n = 6) or 5-AZA (500 ng/side; n = 7). Rats in each group were then tested for PR-STM and PR-LTM 3 and 21 hrs later, respectively, while AEFPs were recorded from the LA. (b) Memory retrieval data during the reactivation session for the vehicle, RG108 and 5-AZA-infused groups. *p<0.05 relative to the pre-CS period. (c) Mean (± SEM) percent freezing during the PR-STM and PR-LTM tests in vehicle, RG108 and 5-AZA-infused groups. *p<0.05 relative to vehicle-infused controls. (d) Mean (± SEM) percent change in AEFP amplitude during the PR-STM and PR-LTM tests in vehicle, RG108 and 5-AZA-infused rats, relative to baseline. *p<0.05 relative to vehicle-infused controls. (e) Correlation between freezing scores and AEFP amplitudes in RG108- and 5-AZA-treated rats during the PR-LTM test, each expressed as a percentage of freezing and AEFP amplitude change during the PR-STM.test. (f) Representative AEFPs recorded from the LA for each group during baseline (light gray trace), PR-STM and PR-LTM sessions (darker traces). Scale bar =10 μV, 5ms.