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. 2014 Oct 21;112(11):1819–1825. doi: 10.1017/S0007114514001998

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© The Authors 2014

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Characterisation of human regulatory T cells (T_reg) enriched from peripheral blood mononuclear cells using immunomagnetic beads. (a) A fluorescence-activated cell sorting-based phenotype analysis of enriched T_reg in lymphocyte gate. Typically, over 94 % of gated CD4⁺CD25⁺ cells expressed the transcription factor forkhead box P3 (FOXP3), a marker for T_reg. (b) High intracellular protein expression of galectin-9 (Gal-9) in stimulated T_reg after 6 d of anti-CD3 and anti-CD28 stimulation. , IgG1-phycoerythrin of stimulated T_reg; , Gal-9-phycoerythrin of stimulated T_reg. PerCP, peridinin chlorophyll; APC, allophycocyanin.

Inline graphic — Characterisation of human regulatory T cells (T_reg) enriched from peripheral blood mononuclear cells using immunomagnetic beads. (a) A fluorescence-activated cell sorting-based phenotype analysis of enriched T_reg in lymphocyte gate. Typically, over 94 % of gated CD4⁺CD25⁺ cells expressed the transcription factor forkhead box P3 (FOXP3), a marker for T_reg. (b) High intracellular protein expression of galectin-9 (Gal-9) in stimulated T_reg after 6 d of anti-CD3 and anti-CD28 stimulation. , IgG1-phycoerythrin of stimulated T_reg; , Gal-9-phycoerythrin of stimulated T_reg. PerCP, peridinin chlorophyll; APC, allophycocyanin.