Figure 2. BN-PAGE analyses of respiratory complexes in Ndufs4^fky/fky cell lines.

Cells derived from +/+ and Ndufs4^fky/fky mice maintained on either glucose or galactose medium (primary MEFs, primary astrocytes), or NBM (primary neurons), were solubilized in buffer containing either 1% Triton X-100 (A and C) or 1% digitonin (B, D), and protein complexes separated by BN-PAGE. Gels were transferred to membrane and immunodecorated with antibody targeted to the CI subunit NDUFA9 (A, B), or targeted to the CIII subunit Core1 (C, D). CII subunit SDHA was used as a loading control. n=1–3. Images have been digitally cropped for clarity. CI–IV, complexes I–IV; Ci, crippled CI formed in the absence of NDUFS4.