Figure 6. Cell death in Ndufs4^fky/fky cell lines.

The propensity of primary MEFs and astrocytes from +/+ and Ndufs4^fky/fky mice to enter cell death was measured by flow cytometry. The ratio of dead cells relative to +/+ (on glucose medium) for primary MEFs is presented in (A) and astrocytes (B), either cultured on glucose medium for 1 day or galactose medium for 1 day or 4 days, with or without acute H₂O₂ treatment for 6 h prior to analysis. The percentage of viable single-celled primary MEFs is presented in (C, E) and astrocytes (D, F), either cultured on glucose for 1 day or galactose for 1 day or 4 days, with (E, F) or without (C, D) acute H₂O₂ treatment for 6 h prior to analysis. Error bars=S.E.M., n≥4, P values generated from two-tailed unpaired t tests correcting for multiple comparisons using the Holm–Sidak method (GraphPad, PRISM V6.0b).*=P≤0.050, **=P≤0.005. PI, propidium iodide.