Table 1. CII-dependent rate of ATP synthesis in isolated mitochondria and permeabilized cells from Ndufs4fky/fky primary cell lines.
*Rate of ATP synthesis measured using the substrate succinate with the complex I inhibitor rotenone±S.E.M. (n). No statistically significant differences were detected (P<0.050 level) between +/+ and fky/fky samples, measured by unpaired t tests, correcting for multiple comparisons using the Holm–Sidak method (GraphPad, PRISM V6.0b).
| ATP (μmol/g/min)* | ||||
|---|---|---|---|---|
| Primary cell line | Medium | Preparation | +/+ | fky/fky |
| MEFs | Glucose | Permeabilzed cells | 36±5.1 (9) | 37±5.0 (13) |
| Galactose | Permeabilized cells | 26±4.1 (7) | 19±4.6 (8) | |
| Glucose | Isolated mitochondria | 61±12 (6) | 65±11 (6) | |
| Astrocytes | Glucose | Permeabilized cells | 61±6.1 (13) | 68±7.6 (11) |
| Galactose | Permeabilized cells | 48±3.9 (7) | 45±1.4 (5) | |
| Glucose | Isolated mitochondria | 84±11 (3) | 89±22 (4) | |
| Neurons | NBM | Permeabilized cells | 24±1.7 (17) | 29±2.3 (21) |