Table 3. Metabolite amounts in RBCs infected with mature trophozoite-stage pfcrt transfectant parasites.
The amounts (μmol per 1012 cells; obtained with 1H-NMR) are from the indicated number of independent cell preparations (obtained on different days), and are shown as means±S.D. The amounts shown are derived from cell preparations with up to 5% contamination with cRBCs. a: P<0.001; b: P<0.01 and c: P<0.05 from FDR-adjusted one-way ANOVA tests using the natural logarithm transformed data (see Table 1). Metabolites for which there were significant differences in abundance among lines are shown in bold.
| ANOVA | C2GC03 | C4Dd2 | C67G8 | |
|---|---|---|---|---|
| No.of independent cultures | 9 | 8 | 7 | |
| CQ resistance status | CQS | CQR | CQR | |
| Amino acids | ||||
| Alanine | b | 15.2±4.5 | 20.8±6.6 | 26.7±3.4 |
| 4-Aminobutyrate | 4.7±2.7 | 4.8±1.4 | 3.6±1.1 | |
| Arginine | c | 24.8±7.5 | 27.9±6.7 | 16.8±6.6 |
| Asparagine | a | 22.6±4.5 | 39.0±8.8 | 42±9 |
| Aspartate | a | 54±5 | 105±30 | 102±26 |
| Glutamate | b | 163±26 | 148±34 | 105±24 |
| Glutamine | a | 44±10 | 66±10 | 79±21 |
| Glycine | a | 12.8±2.5 | 24.1±5.8 | 27.2±1.5 |
| Histidine | b | 14.4±5.9 | 21.2±5.4 | 25.0±5.2 |
| Isoleucine | a | 7.4±1.2 | 10.4±1.2 | 12.3±1.9 |
| Leucine | a | 10.7±1.9 | 18.5±3.8 | 24.3±13.3 |
| Lysine | 31.8±7.5 | 29.1±12.1 | 28.4±6.0 | |
| Phenylalanine | 9.0±6.3 | 8.4±3.6 | 6.5±2.2 | |
| Serine | a | 13.3±3.0 | 22.3±4.0 | 25.3±4.5 |
| Threonine | b | 15.3±2.6 | 20.4±4.4 | 22.4±4.5 |
| Tyrosine | b | 4.4±0.7 | 6.5±1.5 | 7.0±1.3 |
| Valine | b | 6.9±1.0 | 10.2±2.6 | 9.4±1.0 |
| Total amino acids | b | 454±52 | 582±89 | 562±62 |
| Glutathione | ||||
| GSSG | 43±7 | 41±6 | 47±6 | |
| Soluble membrane precursors | ||||
| 2,3-Bisphosphoglycerate | 122±40 | 146±39 | 183±61 | |
| Choline | 69±14 | 70±15 | 80±38 | |
| myo-Inositol | c | 9.4±6.3 | 9.7±1.3 | 15.2±3.4 |
| Phosphocholine | 301±85 | 226±93 | 253±117 | |
| Phosphoethanolamine | 52±15 | 49±20 | 55±23 | |
| Nucleotides and related compounds | ||||
| ADP | 18.0±4.5 | 15.9±2.5 | 19.7±8.6 | |
| ATP | 114±19 | 111±22 | 127±22 | |
| NAD+ | 42±7 | 35.4±12.7 | 51±15 | |
| NADP+ | 4.0±0.7 | 4.4±1.2 | 4.5±0.3 | |
| Carboxylates | ||||
| Acetate | 10.3±9.0 | 7.0±3.8 | 15.6±8.1 | |
| Formate | 8.6±6.2 | 9.1±5.5 | 8.5±4.8 | |
| Fumarate | 1.0±0.4 | 0.8±0.5 | 1.2±0.6 | |
| α-Ketoglutarate | 2.5±0.7 | 3.6±1.1 | 2.6±1.0 | |
| Lactate | 36.3±14.5 | 38.5±10.5 | 43±11 | |
| Malate | 13.3±8.7 | 13.7±3.2 | 10.7±4.5 | |
| Succinate | a | 7.7±1.5 | 4.0±1.7 | 3.7±0.5 |
| Other compounds | ||||
| HEPES | c | 446±39 | 479±92 | 1024±577 |
| Putrescine | 14.3±3.6 | 10.0±5.8 | 9.4±6.0 | |
| Spermidine | c | 144±29 | 101±30 | 87±37 |
| Spermine | a | 9.9±1.9 | 22.2±11.6 | 27.2±9.1 |
| Glucose | 6.3±8.5 | 25.6±31.0 | 38.7±53.9 | |
| Total amounts | 1927±227 | 2005±309 | 2669±851 | |
| Total amounts excluding HEPES | 1481±212 | 1526±255 | 1646±283 | |