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. 2014 Nov 21;34(6):e00153. doi: 10.1042/BSR20140140

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© 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Immunocytochemical staining of Cav-1 in ASMCs obtained from WT mice using an in vitro wound assay (A–D). (A) proliferating cells (white rectangle) proximal to the border of the scratch (yellow dotted line). (B) non-proliferating cells (red rectangle) distal to the border of the scratch. The proliferating and non-proliferating cells are presented in (C) and (D), respectively, at big magnification. Very few Cav-1 positive cells are observed among cells proliferating out into the cell-free area (A, C), whereas confluent, non-proliferating, cells show high Cav-1 immunoreactivity (B, D).