Fig. 1.
(+)-DESI-MS spectra of (a) 5 µM intact insulin solution in water containing 1% formic acid; (b) 5 µM insulin solution after online pepsin digestion and before electrolytic reduction (applied potential: 0 V); and (c) 5 µM insulin solution after online pepsin digestion and online electrolytic reduction (applied potential: −1.5 V).