Table 1.
Characteristics of microRNAs (miRNAs) encoded within the 22q11.2 microdeletion region (current to August 2014).
| miRNA primary coding sequence |
Supporting miRNA evidence and quality |
Target genese |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| miRNA | Coordinates [hg19] | Pub Med No. | miRBase NGS |
Other evidence (miRBase) | GeneCards quality scoreb | Brain expressionc | Conservationd | Predicted target genes | Mean target gene score | |
| Exp. No. | Read No. | |||||||||
| miR-185a | chr22:20020662-20020743 | 27 | 76 | 48635 | Cloned | 3 | Yes | Primate, murine | Yes | 0.042062 |
| miR-649 | chr22:21388465-21388561 | 1 | 3 | 3 | RT-PCR, SAGE | 8 | No | Primate | Yes | 0.099593 |
| miR-1286 | chr22:20236657-20236734 | 1 | 19 | 328 | None | 8 | Yes | Primate | Yes | 0.058791 |
| miR-1306a | chr22:20073581-20073665 | 1 | 61 | 1329 | None | 3 | Yes | Primate, murine | Yes | 0.404076 |
| miR-3618 | chr22:20073269-20073356 | 1 | 8 | 9 | None | 3 | No | Murine | Yes | 0.068121 |
| miR-4761a | chr22:19951276-19951357 | 1 | 12 | 26 | None | 3 | – | None | Yes | 0.080270 |
| miR-6816a | chr22:20102209-20102274 | 1 | 4 | 5 | None | 2 | – | None | No | N/A |
miRNA, miRNAs encoded within the 22q11.2 region (chr22:18876416-21465674 [hg19]); coordinates [hg19], miRNA primary transcript coordinates retrieved from miRBase (release 21) (29); PubMed No., number of PubMed articles retrieved from miRBase (29); Exp No., number of next-generation sequencing (NGS) experiments retrieved from miRBase (29); Read No., read counts from NGS experiments retrieved from miRBase (29); RT-PCR, reverse transcription-polymerase chain reaction; SAGE, serial analysis of gene expression.
a3p and 5p mature miRNA species identified.
bIndicates how many RNA databases have information about this miRNA, and whether the miRNA is expressed or is known to be functional (score ≥5 indicates miRNA is known to be expressed, score ≥10 indicates gene is known to be functional; www.genecards.org).
cHuman prefrontal cortex and/or cerebellar expression (34); mir-4761 and mir-6816 were unavailable for this publication.
dLimited to non-human primate and murine homologs; data collated from miRBase, Ensembl, and NCBI Entrez Gene.
eAvailability of predicted target genes for mature miRNAs with data available using two standard prediction tools (TargetScan and microT-CDS), and average percentile-transformed scores for the 200 top-ranked predicted target genes per miRNA; 149 genes for mir-1306. See text for further details.