Table 5.
Fungi | Distance between the mycelium and wall of oxford cup (cm) | ||||
---|---|---|---|---|---|
9 | M1 | M2 | M4 | M5 | |
VDK | 0.63 ± 0.05d | 0.35 ± 0.03c | 0.13 ± 0.03b | 0.42 ± 0.05c | −0.39 ± 0.04a |
SS | 0.56 ± 0.03d | 0.32 ± 0.03c | 0.54 ± 0.04d | −0.81 ± 0.04a | 0.07 ± 0.03b |
FOC | 0.83 ± 0.03d | −0.87 ± 0.04a | 0.31 ± 0.03b | 0.81 ± 0.02d | 0.60 ± 0.06c |
RSK | 0.39 ± 0.04d | 0.29 ± 0.02c | 0.32 ± 0.03cd | −0.60 ± 0.04a | −0.01 ± 0.04b |
FS | 0.55 ± 0.06c | 0.24 ± 0.07b | 0.14 ± 0.04b | −0.29 ± 0.05a | −0.37 ± 0.05a |
PP | 0.77 ± 0.05b | 0.29 ± 0.03a | 0.25 ± 0.04a | 0.31 ± 0.03a | 0.29 ± 0.04a |
Means and standard deviations were calculated from distances of each mutant classified into confrontation with different fungal pathogens. Small letters in brackets indicate significant differences as determined by One-Way ANOVA and Duncan's multiple range tests.
Antagonistic assay of extractions from SQR9 wild-type and mutant strains against 6 fungi. Data stands for distance between the mycelium and well. VDK, Verticillium dahliae Kleb; SS, Sclerotinia sclerotiorum; FOC, Fusarium oxysporum; RSK, Rhizoctonia solani; FS, Fusarium solani; and PP, Phytophthora parasitica; 9, extraction of SQR9 wild type; M1, extraction of SQR9M1, bacillomycin D deficient mutant; M2, extraction of SQR9M2, fengycin deficient mutant; M4, extraction of SQR9M4, surfactin deficient mutant; and M5, extraction of SQR9M5, bacillibactin deficient mutant. Three replicates were used. Data represent the mean value ± standard error from three replicates. The data obtained were subjected to One-Way ANOVA analysis and means were analyzed by the Duncan's multiple range tests at P ≤ 0.05.Negative value represented that the mycelium growing over the wall of oxford cup and how far it was away from the left wall (of the oxford cup).