Table 3. Secondary antibodies used for immunostaining of 1hippocampal sections, 2hippocampal neurons and 3spinal cord sections.
Antibody | Host | Fluorescent dye | Dilution factor | Company | Catalog nr. |
---|---|---|---|---|---|
α-Rabbit IgG (H+L) 1– 3 | Donkey | Alexa Fluor ® 568 | 1:300 | Molecular probes ® | A-10042 |
α-Mouse IgG (H+L) 1, 2 | Donkey | Alexa Fluor ® 568 | 1:300 | Molecular probes ® | A-10037 |
α-Sheep IgG (H+L) 1, 2 | Donkey | Alexa Fluor ® 568 | 1:300 | Molecular probes ® | A-21099 |
α-Guinea Pig IgG (H+L) 1, 2 | Donkey | CF TM 488A | 1:300 | Sigma | SAB4600033 |
α-Rat IgG (H+L) 1, 2 | Goat | Alexa Fluor ® 568 | 1:300 | Molecular probes ® | A-11077 |
α-Rat IgG (H+L) 3 | Donkey | Alexa Fluor ® 594 | 1:300 | Molecular probes ® | A-21209 |