Table 4.
Flow cytometric analysis A. dealbata seedlings following colchicine treatment of imbibing seeds
| Colchicine treatment | Percentage of seedlings | |||||
| Conc. (mg l–1) | Duration (h) | Germination (%) | 2n* | 3n† | 4n† | Mixoploid* |
| 0 | 50 | 90 | 94·4 | 0 | 5·6 | 0 |
| 0 | 75 | 70 | 100 | 0 | 0 | 0 |
| 0 | 100 | 85 | 94·1 | 0 | 0 | 5·9 |
| 500 | 50 | 60 | 83·3 | 4·2 | 0 | 12·5 |
| 500 | 75 | 60 | 66·7 | 0 | 8·3 | 25·0 |
| 500 | 100 | 60 | 37·5 | 0 | 25·0 | 37·5 |
| 500 | 150 | 53 | 19·1 | 0 | 23·8 | 57·1 |
| 1000 | 50 | 68 | 61·6 | 0 | 19·2 | 19·2 |
| 1000 | 75 | 75 | 63·3 | 0 | 10·0 | 26·7 |
| 1000 | 100 | 58 | 52·2 | 0 | 17·4 | 30·4 |
| 2000 | 50 | 75 | 70·0 | 0 | 6·7 | 23·3 |
| 2000 | 75 | 25 | 80·0 | 0 | 0 | 20·0 |
| 2000 | 100 | 45 | 61·1 | 0 | 22·2 | 16·7 |
| 2000 | 150 | 18 | 42·8 | 0 | 28·6 | 28·6 |
| 3000 | 50 | 83 | 65·6 | 0 | 3·1 | 31·3 |
| 3000 | 75 | 53 | 47·6 | 0 | 28·6 | 23·8 |
| 3000 | 100 | 25 | 30·0 | 0 | 30·0 | 40·0 |
| 3000 | 150 | 53 | 42·9 | 0 | 9·5 | 47·6 |
n = 40 for colchicine treatments, and 20 for controls.
* Identified after 2 months.
† Triploids and tetraploids confirmed after 6 months.