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Fig. 3. Effects of CaCl2 and MgCl2 concentrations in the reaction mixture on the activity of fructokinase extracted from pollen grains (A) and anther tissue (B). The dashed line with open circles represents the effect of CaCl2 in the presence of 0·5 mm MgCl2. Data are means (n = 5) ± s.e. Each assay mixture contained 20 µg protein.