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. 2013 Aug 23;280(19):4876–4887. doi: 10.1111/febs.12463

Figure 1.

Figure 1

Impact of signal peptide on Evasin-4–6His expression. Anti-His western blot analysis of Evasin-4–6His expression in HEK293 cells after transfection using PEI or FuGENE transfection reagent (1 min exposure). Samples were analyzed 6 days post transfection. From the top, expression of: recombinant GlialCam–6His used as positive control, recombinant Evasin-4–6His, recombinant Evasin-4–6His with the uPA signal peptide and recombinant Evasin-4–6His with the Evasin-1 signal peptide. TC, total cells; SN, supernatant; SP, signal peptide.