Figure 3.
Evasin-4 inhibits cell recruitment in vitro. Inhibition of the chemotactic activity of the CC chemokines by increasing concentrations of Evasin-4. (A) Evasin-4 inhibits a broad range of chemokines with IC50 values dependent on the concentration of chemokine used. (B) Evasin-4 is able to inhibit CCL2-mediated chemotaxis, whereas no binding was observed by SPR. (C) By contrast, Evasin-4 does not show any inhibition of CCL1, CCL19, CCL21 and CCL25 but binds to these chemokines by SPR. Data are shown as mean ± SD and are representative of two to seven experiments.