Table 2.
Kinetic characteristics of Evasin-4 binding to CC chemokines by SPR
| Immobilized 6His–Evasin-4 | |||
|---|---|---|---|
| Chemokine | ka × 106 (m−1·s−1) | kd × 10−3(s−1) | KD (nm) |
| CCL1 | 16.6 | 3.26 | 0.20 |
| CCL2 | No binding | ||
| CCL3 | 13.8 | 0.89 | 0.06 |
| CCL4 | No binding | ||
| CCL5 E26A | 26.0 | 2.23 | 0.09 |
| CCL7 | 14.1 | 9.88 | 0.70 |
| CCL8 | 7.11 | 1.76 | 0.25 |
| CCL11 | 12.9 | 4.35 | 0.34 |
| CCL13 | No binding | ||
| CCL14 | 10.4 | 1.48 | 0.14 |
| CCL15 | 40.5 | 93.6 | 2.31 |
| CCL16 | 12.0 | 3.06 | 0.26 |
| CCL17 | 17.6 | 10.7 | 0.61 |
| CCL18 | 19.6 | 0.53 | 0.03 |
| CCL19 | 2.57 | 0.35 | 0.14 |
| CCL20 | No binding | ||
| CCL21 | 11.0 | 0.08 | 0.01 |
| CCL22 | 8.9 | 3.80 | 0.43 |
| CCL23 | 0.006 | 0.03 | 4.43 |
| CCL24 | 30.5 | 12.5 | 0.41 |
| CCL25 | 0.008 | 0.54 | 69.6 |
| CCL26 | 0.17 | 0.15 | 0.88 |
| CCL27 | No binding | ||