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Vestibular primary cell culture. (A) Rat utricles were removed for primary cell culture. Otoconia were gently removed from the utricles and the utricles were gently opened and fixed at the bottom of culture plates. Spreading vestibular cells (arrows) from the utricles were observed after 2 days of incubation. (B-D) Vestibular cells were spindle-shaped, and spreading to whole culture plate. Magnifications were ×40, ×40, ×100, and ×200, respectively.
