Figure 2. The expression of miR-383 and Gadd45g is negatively correlated post UV irradiation.

(A) The mRNA level of Gadd45g was measured by qRT-PCR post UV irradiation (60 J/m²) at indicated time in MCF-7 cells, and normalized to those of GAPDH (top). The mRNA level at 0 h was set as 1.0. The protein level of Gadd45g was measured by western blotting, and β-actin was used as a loading control (bottom). (B) Relative Gadd45g mRNA expression was measured by qRT-PCR 12 h post UV irradiation at different doses (top). The data at 0 J/m² was set as 1.0. Western blotting was used to analyze Gadd45g protein levels. β-actin was used as a loading control (bottom). (C and D) The expression of miR-383 was measured by qRT-PCR post UV irradiation (60 J/m²) at indicated time (C) or different doses (D) in MCF-7 cells, and normalized to the level of U6. The data at 0 h or 0 J/m² was set as 1.0. Values are means ± SD. (E and F) A significant negative correlation was found between miR-383 and Gadd45g expression under UV irradiation post different time or at different doses.