Figure 3. miR-383 regulates cellular apoptotic sensitivity to genotoxic stress through targeting Gadd45g.

(A) MTT assays were performed in miR-383 mimic or a control plasmid transfected MCF-7 cells following treatment with UV irradiation (60 J/m², post 12 h) or cisplatin (25 µM, post 24 h). (B) Apoptosis was analyzed by Annexin V/PI assays in MCF-7 cells transfected with miR-383 mimic or control followed by with UV irradiation or cisplatin treatment. Percentage of apoptotic cells was shown at bottom. (C) MCF-7 cells were co-transfected with miR-383 mimic or control and Gadd45g expression plasmid without the 3′-UTR. After transfection, cells were treated by UV irradiation (60 J/m², post 12 h). Gadd45g levels were reduced by miR-383 mimic, and rescued by the Gadd45g expression plasmid (top). Apoptosis was analyzed by Annexin V/PI assay (bottom). (D) MCF-7 cells were co-transfected with miR-383 mimic or control and Gadd45g expression plasmid without the 3′-UTR, and treated with cisplatin (25 µM, post 24 h). Apoptosis was analyzed by Annexin V/PI assay.