Table 2. The success rate of different primer combinations for the fifteen selected spider species varies from 100% to 30%.
Primer pair | LCO-1490 | LCO-1490 | dgLCO-1490 | dgLCO-1490 | C1-J-2123 | C1-J-2183 | CO1-J-1718 | CO1-RCF1 | CO1-RCF1 | LCO-1490 | ||
HCO-2198 | Chelicerate_R2 | dgHCO-2198 | Chelicerate_R2 | C1-N-2776 | C1-N-2776 | CO1-N-2735 | CO1-RCR1 | CO1-N-2735 | C1-N-2776 | |||
Sample Nr. | Voucher Nr. | Selected Species (see Tree) | ||||||||||
1 | ARA0239 | Agelena labyrinthica | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
2 | ARA0240 | Liphistius sp. | 100% | 100% | 100% | 100% | / | 100% | / | / | / | / |
3 | ARA0111 | Uroctea durandi | 100% | 100% | 100% | 100% | 100% | 100% | 100% | / | / | 50%** |
4 | ARA0120 | Amaurobius erberi | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
5 | ARA0174 | Atypus piceus | 100% | 100% | 100% | 100% | 100% | 100% | / | / | / | / |
6 | ARA0001 | Araneus angulatus | 100%* | 100% | / | 100% | 100% | 100% | 100% | 100% | 100% | / |
7 | ARA0003 | Pholcus phalangioides | 100% | 100% | 100% | / | 100% | / | / | / | / | / |
8 | ARA0004 | Linyphia triangularis | 100% | 100% | 100% | 100% | 100% | 100% | / | / | / | / |
9 | ARA0241 | Hyptiotes paradoxus | 100% | 100% | 100% | 100% | 100% | 100% | 100% | / | / | / |
10 | ARA0242 | Clubiona terrestris | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | / | / |
11 | ARA0243 | Pardosa riparia | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | / |
12 | ARA0029 | Steatoda bipunctata | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | / |
13 | ARA0062 | Evarcha arcuata | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
14 | ARA0244 | Dysdera ninnii | 100% | 100% | 100% | 100% | 100% | 100% | / | 100% | / | / |
15 | ARA0081 | Misumena vatia | 100% | 100% | 100% | 100% | 100% | 100%* | 100% | 100% | 100% | 100% |
Successful in Nr. | 15 | 15 | 14 | 14 | 14 | 14 | 10 | 9 | 7 | 5 | ||
Success Rate | 100% | 100% | 93% | 93% | 93% | 93% | 67% | 60% | 47% | 30% |
Voucher numbers refer to EZ Lab (http://ezlab.zrc-sazu.si/) cryo-collection.
*excised gel band used for 2nd PCR; **only C1-N-2776 binds, one way sequence obtained.