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. 2014 Nov 21;9(11):e113030. doi: 10.1371/journal.pone.0113030

Table 2. The success rate of different primer combinations for the fifteen selected spider species varies from 100% to 30%.

Primer pair LCO-1490 LCO-1490 dgLCO-1490 dgLCO-1490 C1-J-2123 C1-J-2183 CO1-J-1718 CO1-RCF1 CO1-RCF1 LCO-1490
HCO-2198 Chelicerate_R2 dgHCO-2198 Chelicerate_R2 C1-N-2776 C1-N-2776 CO1-N-2735 CO1-RCR1 CO1-N-2735 C1-N-2776
Sample Nr. Voucher Nr. Selected Species (see Tree)
1 ARA0239 Agelena labyrinthica 100% 100% 100% 100% 100% 100% 100% 100% 100% 100%
2 ARA0240 Liphistius sp. 100% 100% 100% 100% / 100% / / / /
3 ARA0111 Uroctea durandi 100% 100% 100% 100% 100% 100% 100% / / 50%**
4 ARA0120 Amaurobius erberi 100% 100% 100% 100% 100% 100% 100% 100% 100% 100%
5 ARA0174 Atypus piceus 100% 100% 100% 100% 100% 100% / / / /
6 ARA0001 Araneus angulatus 100%* 100% / 100% 100% 100% 100% 100% 100% /
7 ARA0003 Pholcus phalangioides 100% 100% 100% / 100% / / / / /
8 ARA0004 Linyphia triangularis 100% 100% 100% 100% 100% 100% / / / /
9 ARA0241 Hyptiotes paradoxus 100% 100% 100% 100% 100% 100% 100% / / /
10 ARA0242 Clubiona terrestris 100% 100% 100% 100% 100% 100% 100% 100% / /
11 ARA0243 Pardosa riparia 100% 100% 100% 100% 100% 100% 100% 100% 100% /
12 ARA0029 Steatoda bipunctata 100% 100% 100% 100% 100% 100% 100% 100% 100% /
13 ARA0062 Evarcha arcuata 100% 100% 100% 100% 100% 100% 100% 100% 100% 100%
14 ARA0244 Dysdera ninnii 100% 100% 100% 100% 100% 100% / 100% / /
15 ARA0081 Misumena vatia 100% 100% 100% 100% 100% 100%* 100% 100% 100% 100%
Successful in Nr. 15 15 14 14 14 14 10 9 7 5
Success Rate 100% 100% 93% 93% 93% 93% 67% 60% 47% 30%

Voucher numbers refer to EZ Lab (http://ezlab.zrc-sazu.si/) cryo-collection.

*excised gel band used for 2nd PCR; **only C1-N-2776 binds, one way sequence obtained.