Figure 5. Western blotting analysis showing the increased MAP2 protein levels in HIV-1_Ba-L (clade B), Cocaine and METH treated and their reversal by Ashwagandha in SK-N-MC neuronal cells.

(A) Cell lysates were separated in 4% to 15% linear gradient SDS-PAGE gels and were probed against the respective antibodies. GAPDH was used as the loading control. (B) Quantitative analysis showing the increased MAP2 protein levels in HIV-1_Ba-L (clade B), Cocaine and METH treated and their reversal by Ashwagandha. The gel shown is a representative for three experiments.