Figure 2. Cyclin B1 overexpression fails to recapitulate effects of vinblastine on phosphorylation of anti-apoptotic Bcl-2 proteins.

HeLa cells were transfected with 2 µg plasmids encoding either wild-type cyclin B1-GFP (Wt), non-degradable cyclin B1-R42A-GFP, or GFP vector for 12–24 h. Untreated untransfected or vinblastine treated (30 nM, 24 h) HeLa cells are shown in the right two lanes. Extracts were prepared and subjected to immunoblotting for the proteins indicated. GAPDH was used as a loading control. Apparent molecular weights (in kDa) are indicated.