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. 2014 Nov 21;9(11):e113789. doi: 10.1371/journal.pone.0113789

Figure 4. Akt inhibition suppresses mTORC1 activity.

Figure 4

BHK-ABCA1 cells were induced overnight with mifepristone (10 nM) and then incubated with DEBC, LY294002 or rapamycin at indicated concentrations for 2 h. Cells were then lysed and analyzed for ABCA1 and phosphorylated S6K by Western blotting. Actin was also blotted as loading control.