Figure 8. Akt inhibition by DEBC decreases membrane lipid rafts defined by density floatation.

ABCA1-expressing BHK cells were untreated (A), treated with 5 mM MCD for 30 min to deplete cholesterol (B) or treated with 5 mM MCD (30 min) and then 5 mM cholesterol/MCD complex (90 min) (C). Some of the cells were also treated with DEBC (50 µM) for 2 h (D). Cells were then collected for density floatation as described in the Methods section. The fractions were then analyzed by Western blotting for flotillin-2. Data is representative of more than three independent experiments.