Figure 9. Akt inhibition by DEBC alters cholesterol packing in the plasma membrane.

A ) BHK-ABCA1 cells were labeled with [³H] cholesterol for one day and induced with mifepristone (10 nM) overnight. Cells were then treated with or without DEBC (50 µM) for 2 h before 30 min incubation with MCD at 0°C. Medium was collected and [³H] cholesterol in the medium was normalized with total cell associated [³H] cholesterol and presented as percentage extraction. B ) BHK-ABCA1 cells were similarly treated as in A, except that MCD extraction was performed at 37°C for 1 min. Data presented as the average of triplicate wells with standard deviation and representative of at least three independent experiments.