. Author manuscript; available in PMC: 2015 Jun 1.

Published in final edited form as: Biomed Chromatogr. 2014 Jun;28(6):729–734. doi: 10.1002/bmc.3177

Table 1.

Estrogenicities of the hop extract and its constituents, 8-PN, 6-PN, XH, and IX, determined using an alkaline phosphatase (AP) induction assay in Ishikawa cells.

Compounds/Extract	AP induction Ishikawa cells EC₅₀	Maximum AP fold induction Ishikawa cells
hop extract [ng/mL] ^a	296.6 ± 8.1	72 ± 8.8
8-PN [nM]	6.65 ± 1.40 ^b	118.0 ± 6.0 ^b
6-PN	Not active^c	Not active^c
XH	Not active	Not active
IX [nM]	634 ± 175	76.8 ± 6.4
17β-estradiol [nM]	0.19 ± 0.05 ^b	137.0 ± 2.5 ^b

Values are expressed as the mean ± SD of at least three independent determinations. Estradiol (0.5 nM) was used as a positive control, and its AP induction was defined as 100-fold.

From Hajirahimhkan, et al. (2013).

From Overk, et al. (2005).