Table 1. Effect of metal ions and chemical reagents on the activity of purified GLA15.
Chemicals | Relative activity (%)a | Chemicals | Relative activity (%) | ||
5 mM | 10 mM | 5 mM | 10 mM | ||
None | 100.0±0.7 | 100.0±1.4 | Cr3+ | 112.9±1.3 | 125.1±0.3 |
Mg2+ | 142.2±0.9 | 135.5±1.6 | Co2+ | 112.3±0.5 | 174.4±1. 7 |
Na+ | 140.4±0.7 | 111.6±1.3 | Li+ | 111.3±1.0 | 132.0±1.5 |
Zn2+ | 140.1±0.4 | 128.6±0.5 | K+ | 110.3±0.5 | 140.4±0.8 |
Cu2+ | 124.2±0.4 | 87.4±1.3 | Mn2+ | 73.0±1.3 | 109.8±1. 6 |
Ca2+ | 116.7±0.3 | 83.7±0.5 | Ag+ | 56.7±1.6 | 36.4±1.4 |
Ni2+ | 115.9±1.0 | 106.9±1.8 | Hg2+ | 12.4±1.4 | 9.8±1.2 |
Fe3+ | 113.9±0.2 | 133.3±0.7 | SDS | 11.4±2.0 | 13.0±1.2 |
Pb2+ | 113.1±1.7 | 118.9±1.4 | β-Mercaptoethanol | 181.8±0.9 | 106.1±1.1 |
Values represent the mean ± SD (n = 3) relative to the untreated control sample.